Filter
Top Products
Doc. 065291-01 3/09 259
F • Glossary
Analytical Column
Synonymous with Separator Column.
Band Spreading
The broadening of the sample band as it travels through the column. Band
spreading can also occur in the injection valve, detector cell, and interconnecting
tubing.
Calibration Curve
A graph showing detector response in peak height or area versus analyte
concentration.
Capacity Factor (k´)
The number of column volumes of eluent, pumped through the column, required
to elute an analyte. Capacity factor is a dimensionless measure of retention which
is independent of column length or eluent flow rate. It is calculated as follows:
Where: t
r
= retention time
t
o
= retention time of unretained solute (column void volume)
k′
t
r
t
o
–
t
o
--------------=