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Doc. 065291-01 3/09 139
3. Open the priming valve by turning it one-quarter to one-half turn
counterclockwise.
4. On the ICS-2100 Control panel in Chromeleon, click the Pump
Settings button. The Pump Settings window opens (see Figure 3-6
).
5. Under Eluent Flow Valve, click the Open button.
6. Draw the syringe back to begin pulling eluent through the flow path.
It may take several syringe draws to remove all air or previous eluent
from the tubing.
NOTE If the vacuum degas assembly is installed, draw
out an additional 20 mL of eluent.
7. After priming the lines thoroughly, close the priming valve. Do not
overtighten.
Figure 5-31. Priming the Eluent Lines
10 mL Syringe
(P/N 054578)
Priming Valve
(open)
Port
Waste Valve
(close)
Primary
Pump Head
Secondary
Pump Head