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Doc. 065291-01 3/09 141
5.17 Priming the Pump with Isopropyl Alcohol
NOTE Prime the pump heads with isopropyl alcohol only if the
two standard priming procedures described in
Section 5.16
are unsuccessful.
1. Connect a 10-mL syringe
(P/N 054578) filled with
isopropyl alcohol (IPA) to
the port in the primary
pump head (see
Figure 5-33
).
2. Open the waste valve on
the secondary pump head
(see Figure 5-33
) by
turning the knob one-
quarter to one-half turn
counterclockwise.
Figure 5-33. Priming the Pump Heads with IPA
3. In the Pump Settings window (see Figure 3-6), click Pump On.
4. Open the priming valve on the primary pump head by turning it one-quarter to
one-half turn counterclockwise.
5. Use the syringe to slowly push alcohol through the pump.
NOTE Be careful not to push any air trapped in the syringe
through the pump. Check the waste line from the
secondary pump head to verify that there are no air
bubbles.
6. Close the priming valve. Do not overtighten. Disconnect the syringe from
the priming valve.
7. Let the pump run for several minutes, to purge alcohol from the pump heads.
Then, click the Prime button in the Pump Settings window to flush the heads
with the desired eluent.
10 mL Syringe
(
P/N 054578
)
Priming Valve
(open)
Port
Waste Valve
(open)
Primary
Pump Head
Secondary
Pump Head