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Doc. 065291-01 3/09 161
Conditioning the EluGen Cartridge
Always condition the EluGen cartridge for 30 minutes before initial use.
1. Make sure the eluent reservoir is filled with ASTM filtered, Type I
(18-megohm) deionized water.
2. Verify that the suppressor and the auxiliary electrolytic device
attached to the
EGC-2 connector (if installed) are off.
3. Verify that the
Automatically turn on with pump option is not
selected on the ICS-2100 touch screen
SUPPRESSOR page (see
Section B.9
). Disabling this option lets you turn on the pump, without
automatically turning on the suppressor.
4. From the Control panel in Chromeleon or the touch screen
HOME
page, set the pump flow rate to 1.00 mL/min.
5. Turn on the pump.
6. From the Chromeleon Control panel or the touch screen
ELUENT
GENERATOR
page, set the EGC-1 concentration to 9mM.
7. Turn on the EGC-1 current.
8. Run at these settings for 30 minutes.
9. Turn off the EGC-1 current.
10. Turn off the pump.
11. Remove the backpressure coil.
12. Reconnect the
TO INJ VALVE IN - P/ELUENT OUT line to the mixer
inlet.
To avoid damaging the suppressor (and auxiliary electrolytic device, if
installed), always turn them off before conditioning the EluGen
cartridge. The pump flow is on during conditioning; however, no flow
reaches the suppressor or auxiliary electrolytic device.