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Doc. 065291-01 3/09 173
5.21.3 Hydrating and Conditioning the EPM
Follow the steps below to hydrate the EPM for 5 minutes and then
condition the EPM for 30 minutes.
1. Fill the eluent reservoir with ASTM filtered, Type I (18-megohm)
deionized water.
2. Verify that the suppressor and the auxiliary electrolytic device (if
installed) are off.
3. Verify that the EluGen cartridge is off.
4. Verify that the
Automatically turn on with pump options are not
selected on the ICS-2100 touch screen
SUPPRESSOR page (see
Section B.9
) and ELUENT GENERATOR page (see Section B.8).
Disabling these options lets you turn on the pump, without
automatically turning on the suppressor and EluGen cartridge.
5. From the ICS-2100 Control panel in Chromeleon or the ICS-2100
touch screen
HOME page, set the pump flow rate to 1.00 mL/min.
6. Turn on the pump.
7. Run the pump for 5 minutes to hydrate the EPM.
8. Select the following settings from the Chromeleon Control panel or
the touch screen
ELUENT GENERATOR page:
• Set the EGC-1 concentration to 9mM.
• Set the EGC-2 concentration to 1mM.
9. Turn on the EGC-1 and EGC-2 currents.
10. Run at these settings for 30 minutes.
11. Turn off the EGC-1 and EGC-2 currents.
12. Turn off the pump.
13. Remove the backpressure coil and couplers from the
TO INJ VALVE IN
- P/ELUENT OUT
and TO SRS/AES REGEN OUT lines.
To avoid damaging the suppressor and auxiliary electrolytic device (if
installed), always turn them off before conditioning the EPM and
EluGen cartridge. The pump flow is on during conditioning; however,
no flow reaches the suppressor or auxiliary electrolytic device.