5
Leica CM3050S – Cryostat
Table of Contents
4. Instrument Properties.............................................................................................................. 21
4.1 Overview ............................................................................................................................................ 21
4.2 Technical Data ........................................................................................................................... 23
5. Operation ................................................................................................................................... 25
5.1 Operating the instrument for the first time ................................................................................... 25
5.2 Switching on/fuses........................................................................................................................... 26
5.3 Control panel 1 .................................................................................................................................. 27
5.3.1 Key functions in control panel 1..................................................................................................... 27
5.3.2 Display functions in control panel 1 .............................................................................................. 27
5.3.3 Display indications when switching on ........................................................................................ 28
5.3.4 Menu functions: setting parameters of refrigeration, time and preset counter .................... 29
5.3.5 Status display.................................................................................................................................... 32
5.3.6 Indication of refrigeration state, section thickness setting and counters .............................. 33
5.4 Control panel 2 .................................................................................................................................. 34
5.4.1 Selecting a sectioning mode / ........................................................................................................ 35
5.4.2 Setting the sectioning window....................................................................................................... 36
5.4.3 Selecting sectioning speed ............................................................................................................ 37
5.4.4 Emergency stop ................................................................................................................................ 37
5.4.5 Coarse feed keys .............................................................................................................................. 38
5.4.6 Trimming and sectioning functions ............................................................................................... 39
5.4.7 Setting the section thickness ......................................................................................................... 39
5.5 Daily operation of the instrument .................................................................................................. 40
5.5.1 Freezing specimens onto specimen .............................................................................................. 40
5.5.2 Freezing specimens onto specimen .............................................................................................. 41
5.5.3 Optional accessories for freezing.................................................................................................. 41
5.5.4 Inserting specimen discs into the.................................................................................................. 42
5.5.5 Specimen orientation....................................................................................................................... 42
5.5.6 Trimming / sectioning ...................................................................................................................... 43
5.6 Finishing work ................................................................................................................................... 44
5.6.1 Daily routine steps............................................................................................................................ 44
5.6.2 Switching off the instrument for a ................................................................................................. 44
5.7 Defrosting .......................................................................................................................................... 45
5.7.1 Automatic chamber defrost cycle ................................................................................................. 45
5.7.2 Manual chamber defrost cycle ...................................................................................................... 46
5.7.3 Defrosting the specimen head ................................................................................................ 46
